From FAT32 to NTFS

Source

To reformat the hard drive from FAT32 to NTFS, follow these directions:To reformat the hard drive from FAT32 to NTFS, follow these directions:
If the drive is the bootable drive:
Backup all data (on all of the hard drives in the computer).
Reboot to the installation CD for the operating system (Windows).
Select the option to reformat the hard drive.
Follow the procedure to reformat the hard drive and reinstall Windows.
If the drive is a storage (secondary) drive:
Backup all data on the drive whose partition will be changed.
Right-click on My Computer.
Select Manage.
Select Disk Management from the left column.
In the right column and bottom window, find the hard drive (labeled, for example, Disk 1).
Locate the partition just to the right of the Disk 1 box.
Right-click on the partition.
Select Delete Partition.
Wait until the partition becomes Unallocated Space.
Right-click on the Unallocated Space.
Select New Partition.
Follow the Create Partition Wizard to create an NTFS partition.
To convert a volume to NTFS from the command prompt

See Microsoft document 307881 for instructions.

Converting the file system from FAT32 to NTFS should be a non-data destructive procedure, however it is always recommended that a current working backup of all data exists before making any changes to the drive.